Cell surface changes in the proteins of rabbit spermatozoa during epididymal passage
作者： Garth L. Nicolson , Adele B. Brodginski , Gillian Beattie , Ryuzo Yanagimachi
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摘要: Differences in the exposure of spermatozoa surface components during epididymal passage have been examined using lactoperoxidase-catalyzed 125I-iodination or labeling with 125I-diazodiiodosulfanilic acid. Labeled proteins obtained from caput and cauda epididymides were solubilized detergent, separated by sodium dodecylsulfate polyacrylamide slab gel electrophoresis, identified radiography. Densitometer scans autoradiograms revealed increased amounts exposures ∼35,000, ∼39,000, ∼50,000, ∼78,000 molecular weight on spermatozoa.
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