The response of human skin commensal bacteria as a reflection of UV radiation: UV-B decreases porphyrin production

摘要: Recent global radiation fears reflect the urgent need for a new modality that can simply determine if people are in risk of developing cancer and other illnesses. Ultraviolet (UV) has been thought to be major factor most skin cancers. Although various biomarkers derived from responses human cells have revealed, detection these is cumbersome, probably requires taking live tissues, varies significantly depending on immune status. Here we hypothesize reaction Propionibacterium acnes (P. acnes), resident commensal, UV serve as early surrogate markers because bacteria immediately responsive radiation. In addition, readily accessible exposed same field body. To test our hypothesis, P. was UV-B The production porphyrins reduced with increasing doses UV-B. porphyrin reduction detected both bacterial isolates. Exposure acnes- inoculated mice led significant decrease single colony simultaneously induced formation cyclobutane pyrimidine dimers (CPD) epidermal layers mouse skin. Mass spectrometric analysis via linear trap quadrupole (LTQ)-Orbitrap XL showed five peptides including an internal peptide (THLPTGIVVSCQNER) chain release 2 (RF2) were oxidized by Seven three 60 kDa chaperonin 1 de-oxidized When compared UV-B, gamma also decreased dose-dependent manner, but different signature protein oxidation/de-oxidation. We highlight uncovering response microbiome will facilitate development pre-symptomatic diagnosis battlefield exposure, nuclear accidents, terrorist attacks, or imaging/therapy.