Promoter analysis of the nuclear gene encoding the chloroplast glyceraldehyde-3-phosphate dehydrogenase B subunit of Arabidopsis thaliana

摘要: The promoter of the nuclear gene, GAPB, which encodes B subunit chloroplast glyceraldehyde-3-phosphate dehydrogenase (GADPH) Arabidopsis thaliana, was previously shown to contain four direct repeats (Gap boxes, located between −237 and −181) that were necessary but not sufficient for light-activated gene transcription. To identify additional elements Gap boxes TATA box, various GAPB fragments driving β-glucuronidase (GUS) reporter constructed in transgenic Arabidopsis. We found a 23 bp element (the XXIII element), centered at −119, is essential activity. Mutations abolished transcription completely. Furthermore, we have identified three elements, PI, Tboxes, PII serve as positive modulators GAPB. any these resulted reduction light inducibility gene. XXIII, Tboxes sequences are novel cis-acting present closely related GAPA or other promoters similarly regulated by light. In our current study, containing promoter::GUS construct with all deleted exhibited significant GUS expression albeit reduced 42% optimal level. contrast, previous studies on tobacco, total abolishment seen when deleted. This suggests different trans-acting factors heterologous systems may result variability transgene.