Evidence that stimulatory factor(s) of RNA polymerase II participates in accurate transcription in a HeLa cell lysate.

作者: K Sekimizu , H Yokoi , S Natori

DOI: 10.1016/S0021-9258(19)81016-5

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摘要: Antibody raised against S-II, a stimulatory factor of RNA polymerase II from Ehrlich ascites tumor cells, inhibited accurate transcription adenovirus 2 major late promoter in HeLa cell lysate. Manganese prevented transcription, but it was essential for expression the activity S-II. The results indicate that protein(s) cross-reacting immunologically with S-II lysate is truncated DNA, this factor(s) not necessary DNA.

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