DLX3 negatively regulates osteoclastic differentiation through microRNA-124

摘要: Homeodomain gene Distal-less-3 (DLX3) plays an essential role in the development of bones. Mutations DLX3 are closely associated with Tricho-Dento-Osseous (TDO) syndrome featured increased bone formation. However, mechanism regarding whether regulates osteoclastogenesis remains largely unknown. In this study, we firstly examined expression mounting during osteoclastic differentiation process, and then established stably expressing wild type (WT-DLX3), a novel mutant (Q178R) found our laboratory recently (MT-DLX3) Dlx3 knockdown cell lines (Dlx3-shRNA) Raw 264.7 cells using corresponding lentiviruses. Next, investigated influence on these stable process osteoclastogenesis. The results showed that osteoclastogenesis-related genes as well tartrate-resistant acid phosphatase-positive multinucleated were lower WT-DLX3 MT-DLX3, but higher Dlx3-shRNA compared control cells. Besides, microRNA-124 was MT-DLX3 Dlx3-shRNA. Moreover, level positively correlated DLX3, negatively NFATc1. Our indicate through microRNA-124, which is partially responsible for density TDO patient. may be exploited regulator potential therapeutic target osteoporosis future.