Biophysical Characterization of a Riboflavin-Conjugated Dendrimer Platform for Targeted Drug Delivery
作者： Amanda B. Witte , Christine M. Timmer , Jeremy J. Gam , Seok Ki Choi , Mark M. Banaszak Holl
DOI: 10.1021/BM201566G
关键词:
摘要: The present study describes the biophysical characterization of generation 5 poly(amidoamine) (PAMAM) dendrimers conjugated with riboflavin (RF) as a cancer targeting platform. Two new series were designed, each presenting ligand attached at different site (isoalloxazine N-3, and D-ribose N-10) varying valency. Isothermal titration calorimetry (ITC) differential scanning (DSC) used to determine binding activity for protein (RfBP) in cell free solution. ITC data shows dendrimer conjugates have KD values ≥465 nM on basis, an affinity ~93-fold lower than that riboflavin. N-3 showed greater comparison N-10 series. Notably, is inversely correlated These findings are also corroborated by DSC where protein-conjugate stability achieved series,
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