Effect of ethanol in vitro and in vivo on Ca2+-activated metabolism of membrane phospholipids in rat synaptosomal and brain slice preparations
作者： M. Hudspith , G.R. John , P.T. Nhamburo , J.M. Littleton
DOI: 10.1016/0741-8329(85)90030-8
关键词:
摘要: Abstract Phospholipase C (PLC) activity was measured by the incorporation of [3H]-inositol into lipids and breakdown [3H]-inositol-labelled phosphatidylinositols (PI) polyphosphatidylinositols (PPI) to phosphates; phospholipase A2 (PLA2) [3H]oleic acid-labelled phosphatidylcholine ([3H]PC) acid enzymes phospholipid base exchange (PLBE) [14C]serine membrane lipids. The activities these in rat brain preparations were all increased procedures which increase intracellular Ca2+, inhibited a varying extent presence ethanol, 50 mM, vitro. In contrast, PLA2 PLBE markedly from animals had received ethanol chronically vivo. Similarly, although basal PLC only slightly such preparations, depolarization induced significantly greater fraction radiolabelled PI than that obtained control preparations. results suggest compensatory alterations Ca2+-activated metabolism tissue during continued
elsevier.com
sci-hub.se 参考文章(26)
Henrik Wallgren, Pirkko Nikander, Pekka Virtanen, Ethanol-Induced Changes in Cation-Stimulated Adenosine Triphospha-Tase Activity and Lipid-Proteolipid Labeling of Brain Microsomes Advances in Experimental Medicine and Biology. ,vol. 59, pp. 23- 36 ,(1975) , 10.1007/978-1-4757-0632-1_3
U Kikkawa, Y Takai, R Minakuchi, S Inohara, Y Nishizuka, Calcium-activated, phospholipid-dependent protein kinase from rat brain. Subcellular distribution, purification, and properties. Journal of Biological Chemistry. ,vol. 257, pp. 13341- 13348 ,(1982) , 10.1016/S0021-9258(18)33453-7
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
M J Berridge, R M C Dawson, C P Downes, J P Heslop, R F Irvine, Changes in the levels of inositol phosphates after agonist-dependent hydrolysis of membrane phosphoinositides. Biochemical Journal. ,vol. 212, pp. 473- 482 ,(1983) , 10.1042/BJ2120473
M J Berridge, C P Downes, M R Hanley, Lithium amplifies agonist-dependent phosphatidylinositol responses in brain and salivary glands Biochemical Journal. ,vol. 206, pp. 587- 595 ,(1982) , 10.1042/BJ2060587
Reiko Natsuki, Robert Hitzemann, Horace Loh, Effects of morphine on the incorporation of [14C]serine into phospholipid via the base-exchange reaction. Molecular Pharmacology. ,vol. 14, pp. 448- 453 ,(1978)
C.D. Richards, J.C. Metcalfe, G.A. Smith, T.R. Hesketh, Changes in free-calcium levels and pH in synaptosomes during transmitter release Biochimica et Biophysica Acta. ,vol. 803, pp. 215- 220 ,(1984) , 10.1016/0167-4889(84)90110-1
Marina A. Lynch, John M. Littleton, Possible association of alcohol tolerance with increased synaptic Ca2+ sensitivity. Nature. ,vol. 303, pp. 175- 176 ,(1983) , 10.1038/303175A0
J. W. Clark, H. Kalant, F. J. Carmichael, Effect of ethanol tolerance on release of acetylcholine and norepinephrine by rat cerebral cortex slices Canadian Journal of Physiology and Pharmacology. ,vol. 55, pp. 758- 768 ,(1977) , 10.1139/Y77-103
T SMITH, Influence of chronic ethanol consumption on muscarinic cholinergic receptors and their linkage to phospholipid metabolism in mouse synaptosomes. Neuropharmacology. ,vol. 22, pp. 661- 663 ,(1983) , 10.1016/0028-3908(83)90160-0