Histology of Cryopreserved Neonatal Rat Testes After Intratesticular Allotransplantation

摘要: Cryopreservation and transplantation of testicular fragments cells are utilized in establishing the germplasm bank endangered species, line-breeding animals. Moreover, tissue is involved into investigation inborn acquired defects tissue. Thus improvement cryopreservation methods great interest. Here, we have compared findings histological examination allotransplanted native cryopreserved neonatal testis. It has been shown that usage DMEM/F12 supplemented with 5 mg/ml bovine serum albumin, 0.05 M sucrose, 20 mM Hepes, 1.5 dimethylsulfoxide as a cryoprotectant, controlled cooling, preservation at -196°C, water bath warming, followed by intratesticular allotransplantation rat testis results development interstitium spermatogenic epithelium donor organs recipient body.   Probl Cryobiol Cryomed 2020; 30(1): 34-46