Targeting lung cancer stem-like cells with TRAIL gene armed oncolytic adenovirus.
作者： Yu Yang , Haineng Xu , Weidan Huang , Miao Ding , Jing Xiao
DOI: 10.1111/JCMM.12397
关键词:
摘要: Lung cancer stem cell (LCSC) is critical in initiation, progression, drug resistance and relapse. Disadvantages showed conventional lung therapy probably because of its existence. In this study, line A549 cells propagated as spheroid bodies (named sphere cells) growth factors-defined serum-free medium. displayed CSC properties, including chemo-resistance, increased proportion G0/G1 cells, slower proliferation rate, ability differentiation enhanced tumour formation vivo. Oncolytic adenovirus ZD55 carrying EGFP gene, ZD55-EGFP, infected inhibited growth. Tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) armed oncolytic adenovirus, ZD55-TRAIL, exhibited cytotoxicity induced apoptosis through mitochondrial pathway. Moreover, small molecules embelin, LY294002 resveratrol improved the ZD55-TRAIL. xenograft models, ZD55-TRAIL significantly survival status mice. These results suggested that gene a potential approach for targeting LCSCs.
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researchgate.net 参考文章(49)
Sri Hari Krishna Vellanki, Andreas Grabrucker, Stefan Liebau, Christian Proepper, Adriana Eramo, Veit Braun, Tobias Boeckers, Klaus-Michael Debatin, Simone Fulda, Small-Molecule XIAP Inhibitors Enhance γ-Irradiation-Induced Apoptosis in Glioblastoma Neoplasia. ,vol. 11, pp. 743- 752 ,(2009) , 10.1593/NEO.09436
Muhammad Alamgeer, Craig D. Peacock, William Matsui, Vinod Ganju, D. Neil Watkins, Cancer stem cells in lung cancer: Evidence and controversies. Respirology. ,vol. 18, pp. 757- 764 ,(2013) , 10.1111/RESP.12094
N Murray, P Coy, J L Pater, I Hodson, A Arnold, B C Zee, D Payne, E C Kostashuk, W K Evans, P Dixon, Importance of timing for thoracic irradiation in the combined modality treatment of limited-stage small-cell lung cancer. The National Cancer Institute of Canada Clinical Trials Group. Journal of Clinical Oncology. ,vol. 11, pp. 336- 344 ,(1993) , 10.1200/JCO.1993.11.2.336
Chris R. Kelsey, Lawrence B. Marks, Donna Hollis, Jessica L. Hubbs, Neal E. Ready, Thomas A. D'Amico, Jessamy A. Boyd, Local recurrence after surgery for early stage lung cancer: an 11-year experience with 975 patients. Cancer. ,vol. 115, pp. 5218- 5227 ,(2009) , 10.1002/CNCR.24625
Maria M. Ho, Alvin V. Ng, Stephen Lam, Jaclyn Y. Hung, Side Population in Human Lung Cancer Cell Lines and Tumors Is Enriched with Stem-like Cancer Cells Cancer Research. ,vol. 67, pp. 4827- 4833 ,(2007) , 10.1158/0008-5472.CAN-06-3557
A Eramo, F Lotti, G Sette, E Pilozzi, M Biffoni, A Di Virgilio, C Conticello, L Ruco, C Peschle, R De Maria, Identification and expansion of the tumorigenic lung cancer stem cell population. Cell Death & Differentiation. ,vol. 15, pp. 504- 514 ,(2008) , 10.1038/SJ.CDD.4402283
M.D. Siegelin, Timo Gaiser, Y. Siegelin, The XIAP inhibitor Embelin enhances TRAIL-mediated apoptosis in malignant glioma cells by down-regulation of the short isoform of FLIP Neurochemistry International. ,vol. 55, pp. 423- 430 ,(2009) , 10.1016/J.NEUINT.2009.04.011
Minna Eriksson, Kilian Guse, Gerd Bauerschmitz, Pekka Virkkunen, Maija Tarkkanen, Minna Tanner, Tanja Hakkarainen, Anna Kanerva, Renee A Desmond, Sari Pesonen, Akseli Hemminki, Oncolytic adenoviruses kill breast cancer initiating CD44+CD24-/low cells. Molecular Therapy. ,vol. 15, pp. 2088- 2093 ,(2007) , 10.1038/SJ.MT.6300300
Xiaochun Zhang, Ritsuko Komaki, Li Wang, Bingliang Fang, Joe Y. Chang, Treatment of Radioresistant Stem-Like Esophageal Cancer Cells by an Apoptotic Gene-Armed, Telomerase-Specific Oncolytic Adenovirus Clinical Cancer Research. ,vol. 14, pp. 2813- 2823 ,(2008) , 10.1158/1078-0432.CCR-07-1528
V Goidts, J Bageritz, L Puccio, S Nakata, M Zapatka, S Barbus, G Toedt, B Campos, A Korshunov, S Momma, E Van Schaftingen, G Reifenberger, C Herold-Mende, P Lichter, B Radlwimmer, RNAi screening in glioma stem-like cells identifies PFKFB4 as a key molecule important for cancer cell survival Oncogene. ,vol. 31, pp. 3235- 3243 ,(2012) , 10.1038/ONC.2011.490