Real-time quantitative LAMP (loop-mediated isothermal amplification of DNA) as a simple method for monitoring ammonia-oxidizing bacteria

摘要: Abstract Loop-mediated isothermal amplification (LAMP) of DNA is a novel technique for the under conditions. For first time, we applied this method to develop simple and quantitative monitoring environmental microorganisms targeting amoA gene in ammonia-oxidizing bacteria. Quantitative analysis was performed by measuring fluorescence derived from an intercalation dye using real-time thermal cycler, then turbidity reaction solution turbidimeter. As result, it possible quantify initial concentration environment with sensitivity down 102 copies target dynamic range 7–9 orders magnitude. Background nontargeted bacteria (Pseudomonas denitrificans) that does not encode did affect capability LAMP. Over results suggested LAMP effective their expression environments.