In vitro proteolytic inhibition, polyphenol oxidase activity, and soluble o-diphenols in grasses and cereals.

摘要: Producing high quality forage remains a challenge due to potential protein degradation during ensiling. Ten grass species and four cereals were screened for proteolytic inhibition, polyphenol oxidase (PPO) activity, the presence of soluble PPO substrates. In orchardgrass, ryegrass, smooth bromegrass, meadow fescue extracts, addition caffeic acid resulted in 70−98% reduction proteolysis both greenhouse field grown environments. These same extracts exhibited 10- 1000-fold higher activity compared all other grasses cereals, depending on substrate supplied environment grown. Chlorogenic two abundant o-diphenols. Tall timothy exhibiting low contained highest levels chlorogenic (3−11 μmol g−1 FW): 100-fold more than any o-diphenol grasses. results suggest that several contain but may lack appropriate substrates effect...