Development of DNA markers that discriminate between white- and blue-flowers in Japanese gentian plants
作者： Takashi Nakatsuka , Misa Saito , Yuka Sato-Ushiku , Eri Yamada , Takashi Nakasato
DOI: 10.1007/S10681-011-0534-7
关键词:
摘要: We developed molecular markers for discrimination of white and blue flower color in Japanese gentian plants. White-flowered gentians can be classified into two types, based on genetic physiological features. One type includes four allelic variations (gtmyb3-1, gtmyb3-2, gtmyb3-3, gtmyb3-4) an anthocyanin biosynthetic regulator gene (GtMYB3), distinguished by three PCR-based markers. The other contains a newly identified inactive allele (ans1) the anthocyanidin synthase (ANS) with premature stop codon generated from 4-bp deletion second exon. ans1 was active ANS cleaved amplified polymorphism sequence (CAPS) marker. genotypes 12 white-flowered cultivars/lines could as either or gtmyb3 using these No contained alleles simultaneously. mutated co-segregated F2 population, demonstrating that CAPS marker is useful to discriminate between flowers gentian. Markers will early selection progeny breeding management.
springer.com
sci-hub.se 参考文章(40)
Shinji Kawasaki, Yasuhiro Murakami, Genome Analysis of Lotus japonicus Journal of Plant Research. ,vol. 113, pp. 497- 506 ,(2000) , 10.1007/PL00013960
Jundae Lee, Jae Bok Yoon, Jung-Heon Han, Won Phil Lee, Sang Hoon Kim, Hyo Guen Park, Three AFLP markers tightly linked to the genic male sterility ms 3 gene in chili pepper (Capsicum annuum L.) and conversion to a CAPS marker Euphytica. ,vol. 173, pp. 55- 61 ,(2010) , 10.1007/S10681-009-0107-1
Andrzej Konieczny, Frederick M. Ausubel, A procedure for mapping Arabidopsis mutations using co‐dominant ecotype‐specific PCR‐based markers Plant Journal. ,vol. 4, pp. 403- 410 ,(1993) , 10.1046/J.1365-313X.1993.04020403.X
Sunggil Kim, Kil Sun Yoo, Leonard M. Pike, Development of a PCR-based marker utilizing a deletion mutation in the dihydroflavonol 4-reductase (DFR) gene responsible for the lack of anthocyanin production in yellow onions (Allium cepa) Theoretical and Applied Genetics. ,vol. 110, pp. 588- 595 ,(2005) , 10.1007/S00122-004-1882-7
S. Kim, R. Jones, K. S. Yoo, L. M. Pike, Gold color in onions (Allium cepa): a natural mutation of the chalcone isomerase gene resulting in a premature stop codon. Molecular Genetics and Genomics. ,vol. 272, pp. 411- 419 ,(2004) , 10.1007/S00438-004-1076-7
Kiyoshi Matsubara, Sumei Chen, Jinxiong Lee, Hiroaki Kodama, Hisashi Kokubun, Hitoshi Watanabe, Toshio Ando, PCR-Based Markers for the Genotype Identification of Flavonoid- 3′, 5′-Hydroxylase Genes Governing Floral Anthocyanin Biosynthesis in Commercial Petunias Breeding Science. ,vol. 56, pp. 389- 397 ,(2006) , 10.1270/JSBBS.56.389
Svetlana Landjeva, Viktor Korzun, Andreas Börner, Molecular markers: actual and potential contributions to wheat genome characterization and breeding Euphytica. ,vol. 156, pp. 271- 296 ,(2007) , 10.1007/S10681-007-9371-0
Takashi Nakatsuka, Masahiro Nishihara, Keiichiro Mishiba, Saburo Yamamura, Two different mutations are involved in the formation of white-flowered gentian plants Plant Science. ,vol. 169, pp. 949- 958 ,(2005) , 10.1016/J.PLANTSCI.2005.06.013
Jean-Marcel Ribaut, David Hoisington, Marker-assisted selection: new tools and strategies Trends in Plant Science. ,vol. 3, pp. 236- 239 ,(1998) , 10.1016/S1360-1385(98)01240-0
Takashi Nakatsuka, Misa Saito, Eri Yamada, Masahiro Nishihara, Production of picotee-type flowers in Japanese gentian by CRES-T Plant Biotechnology. ,vol. 28, pp. 173- 180 ,(2011) , 10.5511/PLANTBIOTECHNOLOGY.10.1101B