作者: Wayne L. Ryan
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摘要: A tissue fixative solution and method is disclosed which comprises a histological fixing composition of diazolidinyl urea, 2-bromo-2-nitropropane-1,3-diol water-soluble zinc salt such as sulfate dissolved in solvent water, alcohol or dimethylsulfoxide. The the invention much less toxic than prior art fixatives thus can be used more safely, conveniently effectively previous solutions techniques. In addition, allows good preservation to obtained, because antigens are retained, useful for all types immunostaining procedures.