On-line post-column fluorescence derivatization of arginine-containing peptides in high-perfomance liquid chromatography
作者： Masahiro Ohno , Masaaki Kai , Yosuke Ohkura
DOI: 10.1016/S0021-9673(01)94275-X
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摘要: Abstract A selective detection system based on on-line post-column fluorescence derivatization is described for the analysis of arginine-containing peptides by high-performance liquid chromatography. The are automatically converted into fluorescent derivatives with benzoin, a fluoregenic reagent guarnidino compounds, after separation reversed-phase column (TSK gel ODS-120T) and in an ultraviolet absorption detector. permits at 435 nm emission irradiation 325 as little picomole amounts. chromatogram obtained fluroscence only shows peaks corresponding to peptides. facile fragments tryptic digest β-melanocyte stimulating hormone model compound could be achieved comparison 215 nm.
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