Essential Notes Regarding the Design of Functional siRNAs for Efficient Mammalian RNAi
作者： Kumiko Ui-Tei , Yuki Naito , Kaoru Saigo
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摘要: Short interfering RNAs (siRNAs) are widely used to bring about RNA interference (RNAi) in mammalian cells. Numerous siRNAs may be designed for any target gene though most of which would incapable efficiently inducing RNAi. Certain highly functional knockout a particular render unrelated endogenous genes nonfunctional. These major bottlenecks should properly eliminated when RNAi technologies employed experiment genomics. This paper thus presents essential notes and findings regarding the proper choice siRNA-sequence selection algorithms web-based online software systems.
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