Influence of the stage for anther excision and heterostyly in embryogenesis induction from eggplant anther cultures

摘要: In this work we address two aspects of eggplant flower biology potentially involved on the efficiency anther culture: selection best floral stage to extract anthers for culture, and effect heterostyly in identification suitable buds anthers. For 12 different accessions, determined morphological criteria (length ranges) identify enriched vacuolate microspores young bicellular pollen, stages most responsive embryogenesis induction. While these microspore/pollen were when isolated cultured liquid medium, observed that culture containing is not choice. Instead, highest response was found younger anthers, mostly mid microspores. We analyzed walls their particular thickness may be behind apparent discrepancy, since they delay diffusion inducing factors locule, reducing over inducible Thus, would allow grow up while are entering locule. also embryogenic short long-styled present Cristal, a heterostylic cultivar. Our results demonstrated each morph produced lengths, but equally useful similar amounts embryos produced. The practical application improve only cultivars, others presenting thick heterostyly.