Functional up-regulation of endopeptidase neurolysin during post-acute and early recovery phases of experimental stroke in mouse brain

摘要: In this study, we provide evidence for the first time that membrane-bound endopeptidase neurolysin is up-regulated in different parts of mouse brain affected by focal ischemia-reperfusion a middle cerebral artery occlusion model stroke. Radioligand binding, enzymatic and immunoblotting experiments membrane preparations frontoparietal cortex, striatum, hippocampus isolated from ischemic hemisphere 24 h after reperfusion revealed statistically significant increase (≥ twofold) quantity activity compared with sham-operated controls. Cerebellar membranes served as negative control supporting observations up-regulation occurs post-ischemic regions. This study also documents sustained functional cortical at least 7 days stroke, which appears not to be transcriptionally or translationally regulated, but rather depends on translocation cytosolic mitochondria. Considering diversity endogenous substrates (neurotensin, bradykinin, angiotensins I/II, substance P, hemopressin, dynorphin A(1-8), metorphamide, somatostatin) well-documented role these peptidergic systems pathogenesis resistance injury and/or post-stroke recovery, our findings suggest may play processes modulating brain's response stroke its recovery We peptidase Neurolysin metabolizes several neuropeptides I II, somatostatin), have various functions pathophysiology. We hypothesize plays key