The Effect of Endogenous and Exogenous Estradiol-17β on the Release of Prostaglandin F2α from the Ovine Uterus

摘要: Utero-ovarian vein blood was collected via indwelling cannulae at 2-hr intervals from 6 conscious cycling ewes, either the uterus in situ or autotransplanted uterus. Radioimmunoassays were used to analyze plasma samples for progesterone (P) estradiol-17β (E2-17β) luteinizing hormone (LH) and prostaglandin F2α (PGF2α-confirined by GLC/mass spec). Although peaks of E2-17β (12 42 pg/ml) occurred throughout estrous cycle (including day 3–4 peak) it not until 13 14 that PGF2α (3 8.5 ng/ml) associated with 132 pg/ml). Larger PGF2 Q (9 23.0 lasting 2 hr less, on 15 completion luteal regression as evidenced a fall P cone. To determine whether association significant also apparent effect PGF2a release direct indirect, physiological amount (1.0 ng/min) was...