Isolation and characterization of an endophytic bacterium, Bacillus megaterium BMN1, associated with root-nodules of Medicago sativa L. growing in Al-Ahsaa region, Saudi Arabia
作者： Ashraf Y. Z. Khalifa , Mohammed A. Almalki
DOI: 10.1007/S13213-014-0946-4
关键词:
摘要: Medicago sativa L. (Alfalfa) is an important forage crop legume in Saudi Arabia due to its high nutritive value and yield. Soil bacteria exist root or root-nodules of either symbiotic relationships associations. In the current study, endophytic bacterium Bacillus megaterium BMN1 was isolated from surface-sterilized characterized phenotypically genotypically. The results indicated that consumed a variety sugars as sole carbon source, produced catalase amylase but not urease. exhibited some plant growth-promoting traits, such production indole acetic acid acetoin, solublization inorganic phosphate. addition, comparative sequence analysis 16S rRNA gene showed 99 % homology with megaterium. could nodulate alfalfa when re-inoculated strain enhanced growth parameters compared uninoculated plants. Co-inoculation BMN1with Sinorhizobium meliloti increased only number nodules formed on roots also length dry weight under greenhouse conditions. Furthermore, effects inoculation Lens esculentus, Phaseolus vulgaris Pisum sativum were assessed. primary root, secondary three legumes significantly upon by control conclusion, belongs group promoting rhizobacteria have significant agricultural applications.
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Alessio Mengoni, Lorenzo Cecchi, Cristina Gonnelli, Nickel Hyperaccumulating Plants and Alyssum bertolonii: Model Systems for Studying Biogeochemical Interactions in Serpentine Soils Springer, Berlin, Heidelberg. pp. 279- 296 ,(2012) , 10.1007/978-3-642-23327-2_14
Alessio Mengoni, Francesco Pini, Marco Bazzicalupo, The Bacterial Flora of the Nickel-Hyperaccumulator Plant Alyssum bertolonii Springer, Dordrecht. pp. 167- 181 ,(2011) , 10.1007/978-94-007-1914-9_7
Leendert C. van Loon, Bernard R. Glick, Increased Plant Fitness by Rhizobacteria Ecological Studies. pp. 177- 205 ,(2004) , 10.1007/978-3-662-08818-0_7
in chief George M. Garrity, Bergey's Manual of Systematic Bacteriology ,(1986)
Konstantinos T. Konstantinidis, Erko Stackebrandt, Defining Taxonomic Ranks Springer Berlin Heidelberg. pp. 229- 254 ,(2006) , 10.1007/978-3-642-30194-0_4
Marina Rodrguez-Daz, Beln Rodelas-Gonzals, Clementina Pozo-Clemente, Maria Victoria Martnez-Toledo, Jess Gonzlez-Lpez, A Review on the Taxonomy and Possible Screening Traits of Plant Growth Promoting Rhizobacteria John Wiley & Sons, Ltd. pp. 55- 80 ,(2008) , 10.1002/9783527621989.CH4
Bart Lambert, Frederik Leyns, Luk Van Rooyen, Francis Gosselé, Yves Papon, Jean Swings, Rhizobacteria of maize and their antifungal activities. Applied and Environmental Microbiology. ,vol. 53, pp. 1866- 1871 ,(1987) , 10.1128/AEM.53.8.1866-1871.1987
Xi-Yang Wu, Mark J. Walker, Michael Hornitzky, James Chin, Development of a group-specific PCR combined with ARDRA for the identification of Bacillus species of environmental significance Journal of Microbiological Methods. ,vol. 64, pp. 107- 119 ,(2006) , 10.1016/J.MIMET.2005.04.021
Syed G. Dastager, Rahul Mawlankar, Shan-Kun Tang, Krishnamurthi Srinivasan, V. Venkata Ramana, Yogesh S. Shouche, Bacillus enclensis sp. nov., isolated from sediment sample Antonie van Leeuwenhoek. ,vol. 105, pp. 199- 206 ,(2014) , 10.1007/S10482-013-0066-3
Martha E Trujillo, Pablo Alonso-Vega, Raúl Rodríguez, Lorena Carro, Eugenia Cerda, Pilar Alonso, Eustoquio Martínez-Molina, The genus Micromonospora is widespread in legume root nodules: the example of Lupinus angustifolius The ISME Journal. ,vol. 4, pp. 1265- 1281 ,(2010) , 10.1038/ISMEJ.2010.55