A new RNA helicase isolated from HeLa cells that catalytically translocates in the 3' to 5' direction.
作者： C.G. Lee , J Hurwitz
DOI: 10.1016/S0021-9258(18)42849-9
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摘要: We have purified an RNA helicase to near homogeneity from nuclear extracts of HeLa cells. The enzyme migrated as a 130-kDa protein upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis and exhibited sedimentation coefficient 6.4 on glycerol gradient centrifugation. translocated in 3' 5' direction acted catalytically, displacing at least 4-fold molar excess duplex compared with the added. All eight common nucleoside triphosphates supported activity relatively low concentrations (Km values 15-20 microM level). In presence some single-stranded DNAs, hydrolyzed all diphosphates inorganic phosphate. displaced deoxyribooligonucleotides provided they were hydrogen-bonded possessing regions, but it did not displace ribooligonucleotides DNA containing regions. enzyme, absence ATP, binds both DNA, amount complex formed was 20-fold greater than DNA. cases, ATP rapidly reversed by addition adenyl-5'-yl (beta,gamma-methylene)-diphosphate. propose that can bind hydrolyze virtue its stability RNA, only translocate
sci-hub.st 参考文章(26)
J A Grifo, R D Abramson, C A Satler, W C Merrick, RNA-stimulated ATPase activity of eukaryotic initiation factors Journal of Biological Chemistry. ,vol. 259, pp. 8648- 8654 ,(1984) , 10.1016/S0021-9258(17)39779-X
B K Ray, T G Lawson, J C Kramer, M H Cladaras, J A Grifo, R D Abramson, W C Merrick, R E Thach, ATP-dependent unwinding of messenger RNA structure by eukaryotic initiation factors. Journal of Biological Chemistry. ,vol. 260, pp. 7651- 7658 ,(1985) , 10.1016/S0021-9258(17)39658-8
A. Claude, J. Arenas, J. Hurwitz, The isolation and characterization of an RNA helicase from nuclear extracts of HeLa cells. Journal of Biological Chemistry. ,vol. 266, pp. 10358- 10367 ,(1991) , 10.1016/S0021-9258(18)99234-3
Y.S. Seo, S.H. Lee, J. Hurwitz, Isolation of a DNA helicase from HeLa cells requiring the multisubunit human single-stranded DNA-binding protein for activity. Journal of Biological Chemistry. ,vol. 266, pp. 13161- 13170 ,(1991) , 10.1016/S0021-9258(18)98819-8
J A Grifo, S M Tahara, J P Leis, M A Morgan, A J Shatkin, W C Merrick, Characterization of eukaryotic initiation factor 4A, a protein involved in ATP-dependent binding of globin mRNA. Journal of Biological Chemistry. ,vol. 257, pp. 5246- 5252 ,(1982) , 10.1016/S0021-9258(18)34662-3
R. D. Iggo, D. P. Lane, Nuclear protein p68 is an RNA-dependent ATPase. The EMBO Journal. ,vol. 8, pp. 1827- 1831 ,(1989) , 10.1002/J.1460-2075.1989.TB03577.X
R Pruzan, H Furneaux, P Lassota, G Y Hong, J Hurwitz, Assemblage of the prespliceosome complex with separated fractions isolated from HeLa cells. Journal of Biological Chemistry. ,vol. 265, pp. 2804- 2813 ,(1990) , 10.1016/S0021-9258(19)39873-4
E J Strauss, C Guthrie, A cold-sensitive mRNA splicing mutant is a member of the RNA helicase gene family. Genes & Development. ,vol. 5, pp. 629- 641 ,(1991) , 10.1101/GAD.5.4.629
Martin Scheffner, Rolf Knippers, Hans Stahl, RNA unwinding activity of SV40 large T antigen Cell. ,vol. 57, pp. 955- 963 ,(1989) , 10.1016/0092-8674(89)90334-6
Catherine A. Brennan, Alicia J. Dombroski, Terry Platt, Transcription termination factor rho is an RNA-DNA helicase Cell. ,vol. 48, pp. 945- 952 ,(1987) , 10.1016/0092-8674(87)90703-3