IHF-independent assembly of the Tn10 strand transfer transpososome: implications for inhibition of disintegration

摘要: The frequency of DNA transposition in systems that employ a strand transfer step may be significantly affected by the occurrence disintegration reaction, reaction reverses event. We have asked whether occurs Tn10 system. show substrates (substrates constituting one half product) are assembled into transpososome mimics intermediate. This (STT) does appear to support an intermolecular but only at very low level. Strikingly, assembly STT is not dependent on IHF, host protein required for de novo all previously characterized transpososomes. suggest able form both transposon end and target type contacts with transposase because their enhanced conformational flexibility. probably allows conformation within complex prevents destructive responsible relaxing sequence requirements formation relative other