Chlorine affects photosystem II and modulates the transcriptional levels of photosynthesis-related genes in the dinoflagellate Prorocentrum minimum

摘要: Chlorine (Cl2) is a commonly used biocide; however, the cellular mechanism and pathways associated with Cl2 exposure in algae have not been elucidated. Here, we investigated physiological gene transcriptional responses dinoflagellate Prorocentrum minimum upon chlorination, emphasis on photosynthetic system. The time-course stress response of P. was assessed by measuring transcription photosynthesis-related genes, chlorophyll autofluorescence (CAF), photosynthesis efficiency (F v /F m ), to series doses (0.1, 0.5, 1.0 mg L−1). CAF data showed marked reduction fluorescence intensity increasing time (0, 6, 12, 24 h). F dramatically decreased after 1 mg L−1 exposure. Transcriptional photosystem related genes (PmpsbA, PmatpB, PmrbcL, PmpsaA) were analysed using quantitative real-time (qRT)-PCR. PmpsbA significantly upregulated 0.5 1.0 mg L−1 24 h However, expressions PmatpB PmpsaA downregulated. Our results suggest that such as may act indicators environmental can be useful rapid biomarker candidates for ecotoxicogenomic assessments.