The characterization and synthesis of antigens related to factor VIII in vascular endothelium.
作者： Sonia A.M. Shearn , I.R. Peake , J.C. Giddings , J. Humphrys , A.L. Bloom
DOI: 10.1016/0049-3848(77)90067-6
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摘要: Abstract Umbilical cord endothelial cells were obtained by collagenase digestion and maintained in culture. Release of factor VIII related antigen (FVIIIRAG) ristocetin co-factor activity demonstrated specific assays. Synthesis FVIIIRAG was confirmed incorporation 35S L-methionine into immunoprecipitate two dimensional crossed immunoelectrophoresis (2DCIE) autoradiography. synthesised had increased anodal electrophoretic mobility compared to that normal adult blood. procoagulant (FVIIIC) cultured not coagulation assay. A rabbit antiserum FVIIIRAG/FVIIIC rendered non precipitating apparently monospecific for FVIIIC absorption with immobilised dissociated FVIIIRAG. Using this antigenic determinants detected immunohistological means or vascular intima. The results suggest the released a modified, incomplete precursor form VIII.
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