Analysis of Gene Expression Patterns and Chromosomal Changes Associated with Aging

摘要: Age is the largest single risk factor for development of cancer in mammals. Age-associated chromosomal changes, such as aneuploidy and telomere erosion, may be vitally involved initial steps tumorigenesis. However, changes gene expression specific increased with age have not yet been characterized. Here, we address these questions by using a panel fibroblast cell lines lymphocyte cultures from young old groups. Oligonucleotide microarrays were used to characterize 14,500 genes. We measured length analyzed chromosome copy number structural rearrangements multicolor interphase fluorescence situ hybridization 7-fluorochrome multiplex hybridization, tried show relationship between patterns changes. These analyses revealed genes both cycle proliferation that are differently expressed aged cells. More importantly, our data an association age-related level centromere kinetochore function microtubule spindle assembly apparatus. To verify some also tumorigenesis, compared chromosomally stable microsatellite instability unstable colorectal tumor lines. Three (Notch2, H2AFY2, CDC5L) showed similar differences observed suggesting they play role