Purification of avian biological material to refined keratin fibres

摘要: Keratin derived from chicken feather fibres (CFFs) has many potential applications that are constrained by the quality and pathogen content after purification treatment. The activity not been evaluated elsewhere. Plucked feathers prone to impose biological hazards due accommodating blood-borne pathogens; therefore, establishing an efficient process is crucial. Bactericidal performance of surfactants (anionic, non-ionic, cationic), bleach (ozone chlorine dioxide), ethanol extraction, a combination method comprising surfactant-bleach-ethanol extraction on was investigated via (A) standard plate count enumeration Escherichia coli, Pseudomonas species, coagulase positive Staphylococcus, aerobic anaerobic spore-formers (B) Salmonella Campylobacter detection tests. Among methods, only methods eliminated untreated feathers. Although exhibited superior bactericidal impact compared method, treated through latter demonstrated morphological mechanical properties. Scanning electron microscopy-energy dispersive spectroscopy employed determine remaining selected purifiers CFFs. Fourier-transform infrared confirmed successful removal fatty esters CFFs using nominated purifiers. Ethanol found be most efficacious single treatment, while surfactant oxidative sterilizer with superior.