Dihydroartemisinin increases temozolomide efficacy in glioma cells by inducing autophagy.

摘要: Artemisinin, a powerful antimalarial medicine, is extracted from the Chinese herb, Artemisia annua L., and has ability to inhibit proliferation of cancer cells. Dihydroartemisinin (DHA), major active metabolite artemisinin, able growth variety types human cancer. However, effect DHA on glioma cells remains unclear. The aim present study was investigate cells, whether enhance temozolomide (TMZ) sensitivity in vitro vivo. In total, 10 cell lines were used analyze inhibition by MTT assay. typical autophagic vacuoles monitored application autofluorescent agent, monodansylcadaverine. Western blotting detect markers apoptosis autophagy, namely Caspase-3, Beclin-1 LC3-B. combination efficiency TMZ assessed half maximal inhibitory concentration (IC50) differed among ten lines. number higher DHA-treated SKMG-4 cells; this highest all analyzed. expression autophagy molecular markers, LC3-B, increased following treatment, while no significant alteration detected apoptotic marker Caspase-3. When combined with DHA, IC50 decreased significantly four Furthermore, enhanced tumor tumor-burdened mice. results demonstrated that inhibited efficacy vivo through induction autophagy.