Concomitant BRAFV600E Mutation and RET/PTC Rearrangement Is a Frequent Occurrence in Papillary Thyroid Carcinoma

摘要: Background: The tyrosine kinase receptors/RAS/RAF/MAPK cascade is a site of mutational events associated with thyroid carcinogenesis. Some studies suggest the reciprocal exclusion different oncogenes in mitogen-activated protein cascade, whereas others that BRAF mutations and RET rearrangements can simultaneously occur sporadic cases. aim this study was to determine prevalence concomitant BRAFV600E mutation RET/PTC same tumor its association some clinicopathological features. Methods: percentage mutant alleles presence were determined by means pyrosequencing Southern blot analysis reverse transcription polymerase chain reaction products series 72 conventional papillary carcinomas (PTCs). Then, associations between characteristics status assessed. Results: present 32 out PTCs (44.4%) the...