Transferrin-conjugated quasi-cubic SPIONs for cellular receptor profiling and detection of brain cancer

摘要: Abstract The high biocompatibility, unique magnetic properties and significant biomedical potential of superparamagnetic iron oxide nanoparticles (SPIONs) have spurred a major interest in developing new strategies to synthesise aqueous-phase dispersible SPIONs. In parallel, the rapidly growing field enzyme-mimicking catalytic nanomaterials, referred as NanoZymes, has seen immense utilising NanoZymes for applications. Here, we report strategy large-scale synthesis water quasi-cubic γ-Fe2O3 This provides way control morphological characteristics SPIONs through in-situ formation sodium oleate complex. Notably, our approach eliminates need temperatures, complex cleaning protocols post-synthesis phase-transfer that are typically required during well-established thermal decomposition approaches. We further establish peroxidase-mimic NanoZyme activity these use them an effective transducer platform generate colour efficient oxidation peroxidase substrates. By combining inorganic enzymes with transferrin (Tf) brain cancer-specific targeting agent, create simple yet colorimetric biosensing allows us monitor cellular expression profile receptors (TfR). difference TfR U87MG glioblastoma cells fibroblast generates distinct tonality response. not only cell surface receptor profiling but also robust identification cancer cells. accuracy precision proposed biosensor outlines robustness NanoZyme-based catalysts detection various targets.