Shedding new light on lipid biology with coherent anti-Stokes Raman scattering microscopy

摘要: Despite the ubiquitous roles of lipids in biology, detection has relied on invasive techniques, population measurements, or nonspecific labeling. Such difficulties can be circumvented by a label-free imaging technique known as coherent anti-Stokes Raman (CARS) microscopy, which is capable chemically selective, highly sensitive, and high-speed lipid-rich structures with submicron three-dimensional spatial resolution. We review broad applications CARS microscopy to studies lipid biology cell cultures, tissue biopsies, model organisms. Recent technical advances, limitations technique, perspectives are discussed.