Amniotic Fluid Stem Cell Populations

摘要: Amniotic fluid can be considered not only a precious source of information about the developing embryo/fetus, but also reservoir potentially invaluable cells for regenerative medicine. fluid-derived fulfill two criteria to clinically appealing: (1) they routinely collected as side product amniocenteses with no ethical issues, and (2) easily expanded in vitro preparation autologous transplantation newborn period. Amniocytes have variable morphology expression markers from all three germ layers, making it difficult determine their ontology. Gestational age, health status fetus, compartment origin, isolation methods are variables that contribute heterogeneity this population. In last decade several efforts has been done order identify characterize subpopulations progenitor/stem-like cells. Fluid Mesenchymal Stromal Cells (AF-MSCs) related bone marrow counterpart, however replicate at higher rate more accessible fetus. Due polyclonal nature, variability terms pluripotency differentiation potential reported. Stem (AFSCs), on other hand, clonal population rare broader multipotency. Increased standardization cell isolation, culture, preservation well detailed characterization continue evolve prerequisites towards eventual clinical application AF-MSCs AFSCs patients.