Genome-wide analysis of epigenetic signatures for kidney-specific transporters.

摘要: DNA methylation-dependent gene silencing is one of the most characterized mechanisms in epigenetic regulation expression. This process thought to influence ability hepatocyte nuclear factor 1 (HNF1) transactivate organic anion transporter expression liver and kidney. To evaluate this further we profiled 282 mouse solute carrier transporters by examining regions near their transcription start sites for tissue-dependent differentially methylated (T-DMR) using restriction tag-mediated amplification determine T-DMR disparity between Forty-two these were associated with tags hypomethylated kidney but hypermethylated liver. Computational analysis found a canonical HNF1-binding motif within 1kbp promoter region 13 carriers including amino acid Slc6a19 , Slc6a20 Slc7a8 Slc7a9 ; all expressed predominantly Bisulfite genomic sequencing that CpG dinucleotides neighboring compared The Hnf1 α itself contained cerebrum, consistent tissue distribution Hnf1α. Taken together, our results show central role methylation kidney-specific thus determining both master regulator, Hnf1α, its interaction downstream genes.