The Mechanism of Particulate Carrier Reactions I. Adsorption of Human γ-Globulin to Polystyrene Latex Particles

摘要: Summary Suspensions of polystyrene latex particles treated with human γ-globulin are empolyed in a variety serologic agglutination procedures. The protein is adsorbed onto the surface and this adsorption can be described by Langmuir type isotherm. process two steps. amount first step proportional to area particles. data obtained consistent interpretations. One that as double molecular layer where second contains about half layer. An alternate view forms random immobile film molecules oriented parallel particle surface. Further then takes place interstices between already adsorbed, but now perpendicularly Estimations fraction occupied different orientations good agreement theoretic calculations. A 8020 diameter adsorb maximum 75,000 molecules.