Butyrate and retinoic acid imprint mucosal-like dendritic cell development synergistically from bone marrow cells.

摘要: Accumulating data show that the phenotypes and functions of distinctive mucosal dendritic cells (DCs) in gut are regulated by retinoic acid (RA). Unfortunately, exact role butyrate RA-mediated DC differentiation has not been elucidated thoroughly to date. Mucosal-like cell was completed vitro culturing bone marrow with growth factors [granulocyte-macrophage colony-stimulating factor (GM-CSF/interleukin (IL)-4], RA and/or butyrate. The phenotypes, cytokine secretion, immune levels retinal dehydrogenase different DCs were detected using quantitative polymerase chain reaction (qPCR), enzyme-linked immunosorbent assay (ELISA) flow cytometry, respectively. results showed RA-induced (RA-DCs) properties, including expression CD103 homing receptor α4 β7 , low proinflammatory secretion priming capability antigen-specific CD4+ T cells. Butyrate-treated RA-DCs (Bu-RA-DCs) decreased CD11c, but increased expression. Moreover, suppressed significantly Thus, have synergistic regulatory on differentiation, indicating homeostasis depends largely upon imprint subsets, both individually collectively.