Ultrasound-mediated tumor imaging and nanotherapy using drug loaded, block copolymer stabilized perfluorocarbon nanoemulsions.

摘要: Perfluorocarbon nanoemulsions can deliver lipophilic therapeutic agents to solid tumors and simultaneously provide for monitoring nanocarrier biodistribution via ultrasonography and/or (19)F MRI. In the first generation of block copolymer stabilized perfluorocarbon nanoemulsions, perfluoropentane (PFP) was used as droplet forming compound. Although manifesting excellent ultrasound imaging properties, PFP were unstable at storage, difficult handle, underwent hard control phenomenon irreversible droplet-to-bubble transition upon injection. To solve above problems, perfluoro-15-crown-5-ether (PFCE) a core compound in second nanoemulsions. PFCE nanodroplets manifest both fluorine ((19)F) MR contrast which allows using multimodal spectroscopy nanodroplet pharmacokinetics biodistribution. present paper, acoustic, imaging, properties unloaded paclitaxel (PTX) loaded are reported. As manifested by spectroscopy, long circulating, with about 50% injected dose remaining circulation 2h after systemic Sonication 1-MHz triggered reversible Microbubbles formed acoustic vaporization stable cavitation. The size (200nm 350nm depending on type shell conditions emulsification) well residence favored their passive accumulation tumor tissue that confirmed ultrasonography. breast pancreatic cancer animal models, ultrasound-mediated therapy paclitaxel-loaded showed characterized regression suppression metastasis. Anticipated mechanisms observed effects discussed.