Development and evaluation of an enzyme-linked immunosorbent assay using recombinant p23 for the detection of antibodies to Cryptosporidium parvum in cattle
作者： 学南 玄 , 義文 西川 , Xuenan Xuan , 郁男 五十嵐 , Hiroshi Bannai
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摘要: An enzyme-linked immunosorbent assay (ELISA) based on the recombinant p23 of Cryptosporidium parvum was established for detection antibodies against C. in cattle. The sensitivity and specificity ELISA were evaluated with standard indirect fluorescent antibody test (IFAT) using sporozoites as antigens. Of 77 bovine sera collected from China, 20 (26.0%) identified positive by IFAT. same samples tested ELISA. optical densities at 415 nm compared to IFAT results statistically analyzed designate a provisional cut-off point. As result, point concluded be 0.08, which considered best condition light its (80%) (73.7%). Thus, it demonstrated that could useful tool diagnosis infection cattle under field conditions estimate magnitude asymptomatic population subsequent risk humans.
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