Cytogenetic localization by variation in electrophoretic allozyme phenotype: Drosophila Odh.

摘要: A gene-dosage effect is characteristic of eukaryotic structural genes and therefore useful in gene mapping. However, attributing quantitative variations enzyme activity to a or other putative regulatory loci can be suspect when the locus question may inducible by culture conditions. The problem controlling for allele-specific regulation circumvented Drosophila melanogaster use synthetic duplications deficiencies conjunction with polymorphism. method constructing segmental aneupliods heterozygous electrophoretic variants octanol dehydrogenase (Odh) presented which permitted allozyme phenotype activity—which show strict dosage dependency—to produced simultaneously. region Odh was identified using T(Y;A) stocks deficiency M(3)S31 used assign polytene band 86D1–4. With this aneuploid survey purposes localization accomplished one generation appropriate stocks.