Cell cycle regulation of metallothionein in human colonic cancer cells.

摘要: Elevated levels of metallothionein (MT) found in rapidly growing tissues such as neonatal liver and various types human tumors have suggested a role for MT cell proliferation. To further explore this possibility we investigated the concentration colonic cancer (HT-29) cells at different stages proliferation by means immunocytochemistry competitive binding. is increased subconfluent proliferating relative to growth-inhibited confluent cells, much it tissues. Cycling synchronized with compactin, an inhibitor 3-hydroxy-3-methylglutaryl-coenzyme A reductase, revealed oscillation cytoplasmic that reached maximum successive late G1 phases G1/S transition. Individual phase cycle were assessed [3H]thymidine incorporation immunofluorescence employing antibody detects nuclear antigen associated An enzyme-linked immunosorbent assay was used quantify amounts homogenate supernatants HT-29 cells. 2- 3-fold increase actively regulation protein during mitotic point physiological cellular suggest may also serve marker.