CdeA of Clostridium difficile, a new multidrug efflux transporter of the MATE family.
作者： L. Dridi , J. Tankovic , J.-C. Petit
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摘要: The cdeA gene, cloned from Clostridium difficile clinical strain 714 under the control of its natural promoter made Escherichia coli and perfringens resistant to ethidium bromide acriflavin but had no effect on susceptibility hosts following antibiotics: norfloxacin, ciprofloxacin, gentamicin, erythromycin, tetracyclin, chloramphenicol. However, it was responsible for fluoroquinolone resistance in E. when Plac promoter. Quantitative reverse transcriptase (RT)- PCR showed that growth C. 253 presence subinhibitory concentrations significantly increased transcription cdeA, this not observed with ciprofloxacin. deduced protein homologous sequences known efflux pumps third cluster (the so-called DinF branch) multidrug toxic compound extrusion (MATE) family. CdeA caused energy-dependent in...
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