Solution-state behaviour of algal mono-uronates evaluated by pure shift and compressive sampling NMR techniques.

摘要: Sodium salts of the algal uronic-acids, d-mannuronic acid (HManA) and l-guluronic (HGulA) have been isolated characterised in solution by nuclear magnetic resonance (NMR) spectroscopy. A suite recently-described NMR experiments (including pure shift compressive sampling techniques) were used to provide confident assignments pyranose forms two uronic acids at various pD values (from 7.5 1.4). The resulting high resolution spectra determine several previously unknown parameters for acids, including their pKa values, position isomeric equilibria, propensity form furanurono-6,3-lactones. For each three parameters, comparisons are drawn with behaviour related D-glucuronic (HGlcA) D-galacturonic (HGalA), which studied extensively. This paper demonstrates how these new spectroscopic techniques can be applied better understand properties polyuronides uronide-rich macroalgal biomass.