TIME-RESOLVED FLUORESCENCE-DECAY MEASUREMENT AND ANALYSIS ON SINGLE CELLS BY FLOW CYTOMETRY
作者： Chiranjit Deka , John A. Steinkamp
DOI: 10.1364/AO.35.004481
关键词:
摘要: A novel method is described for the measurement and analysis of fluorescence decays individual cells particles in flow. It combines rapid capabilities a flow cytometer robust procedures time-domain fluorescence-lifetime spectroscopy. For excitation we use cw laser that pulse modulated by an electro-optic modulator. The characteristics repetition rate pulses can be easily adjusted to accommodate with wide range lifetimes.
osapublishing.org
harvard.edu参考文章(20)
TH. FÖRSTER, Mechanisms of Energy Transfer Comprehensive Biochemistry. ,vol. 22, pp. 61- 80 ,(1967) , 10.1016/B978-1-4831-9712-8.50010-2
Raymond F. Chen, Jay R. Knutson, Mechanism of fluorescence concentration quenching of carboxyfluorescein in liposomes: energy transfer to nonfluorescent dimers. Analytical Biochemistry. ,vol. 172, pp. 61- 77 ,(1988) , 10.1016/0003-2697(88)90412-5
William R. Ware, Laurence J. Doemeny, Thomas L. Nemzek, Deconvolution of fluorescence and phosphorescence decay curves. Least-squares method The Journal of Physical Chemistry. ,vol. 77, pp. 2038- 2048 ,(1973) , 10.1021/J100636A003
Steven A. Soper, Benjamin L. Legendre, Error Analysis of Simple Algorithms for Determining Fluorescence Lifetimes in Ultradilute Dye Solutions Applied Spectroscopy. ,vol. 48, pp. 400- 405 ,(1994) , 10.1366/0003702944028191
Dennis P. Heller, Clive L. Greenstock, Fluorescence lifetime analysis of DNA intercalated ethidium bromide and quenching by free dye Biophysical Chemistry. ,vol. 50, pp. 305- 312 ,(1994) , 10.1016/0301-4622(93)E0101-A
E. Gratton, R. Lopez-Delgado, Measuring fluorescence decay times by phase-shift and modulation techniques using the high harmonic content of pulsed light sources Il Nuovo Cimento B. ,vol. 56, pp. 110- 124 ,(1980) , 10.1007/BF02738361
Hualing Zeng, Gilles Durocher, Analysis of fluorescence quenching in some antioxidants from non-linear Stern-Volmer plots Journal of Luminescence. ,vol. 63, pp. 75- 84 ,(1995) , 10.1016/0022-2313(94)00045-E
S.M. Keating, T.G. Wensel, Nanosecond fluorescence microscopy. Emission kinetics of fura-2 in single cells. Biophysical Journal. ,vol. 59, pp. 186- 202 ,(1991) , 10.1016/S0006-3495(91)82210-X
Joseph R. Lakowicz, Gabor Laczko, Ignacy Gryczynski, Picosecond resolution of tyrosine fluorescence and anisotropy decays by 2-GHz frequency-domain fluorometry. Biochemistry. ,vol. 26, pp. 82- 90 ,(1987) , 10.1021/BI00375A012
D.M. Rayner, A.E. McKinnon, A. G. Szabo, Correction of instrumental time response variation with wavelength in fluorescence lifetime determinations in the ultraviolet region Review of Scientific Instruments. ,vol. 48, pp. 1050- 1054 ,(1977) , 10.1063/1.1135182