Characterization of human LNX, a novel ligand of Numb protein X that is downregulated in human gliomas

摘要: Gliomas are major tumors of the central nervous system with a wide spectrum different tumor types. Ligand Numb protein X (LNX) is PDZ domain containing that interacts cell fate determinant Numb. cDNA microarray analysis was used to determine expression 13,939 genes in set 18 gliomas. It showed human LNX downregulated 100% gliomas including low- and high-grade ones, which confirmed by Northern blot. In situ hybridization revealed lowly expressed cytoplasm glioma cells. Thus, might act as diagnostic marker potential therapeutic target for glioma. Two-hybrid screen yeast identify interacting proteins important function. interacted Ski (SKIP) via domains. The co-immunoprecipitation results suggested SKIP HEK293 could affect subcellular localization Numb, indicated function molecular anchor localized site its interaction Notch. presence multiple binding domains involved signal transduction an role tumorogenesis.