Sexing of porcine embryo by in situ hybridization using chromosome Y- and 1-specific DNA probes.

摘要: Abstract This study was carried out to determine if a rapid, simultaneous detection system using chromosome Y- and 1-bearing boar spermatozoa applicable for sexing embryos. Porcine embryos were recovered from gilts sows 4 6 d after mating, whole or biopsy cells mounted on glass slide with small amount of fixative (methanol: acetic acid: distilled water = 9:1:4). The samples then stained by means fluorescence in situ hybridization (FISH) procedure developed specifically the Y-bearing spermatozoa. Hybridization performed digoxigenin (dig)-labeled specific DNA, biotin-labeled 1-specific DNA sequences detected as signal FITC Texas Red nucleus visualized DAPI-stain. Proportions labeled 1-probe 17 97% at 3 16 ≧32 cell stage, respectively. Of 93 biopsied analyzed FISH, 85 (91 %) could be accurately classified male female. 65 embryos, 60 (92%) had clear blastocoele inner mass 48 h culture vitro, these evaluated available One recipient which received sexed transfer farrowed 12 piglets expected sex. results this demonstrated that porcine stage can within 2 FISH method. Moreover further development technique make it an effective tool early control