Co-operative interactions between NFI and the adenovirus DNA binding protein at the adenovirus origin of replication.

摘要: The DNA-protein and protein-protein interactions proposed for the stability of nucleoprotein complexes at origin replication in prokaryotes are also thought to impart regulatory precision eukaryotic DNA replication. This type specificity can be observed, example, during adenovirus where efficient initiation requires that nuclear factor I (NFI) binds Addition purified NFI stimulates vitro a reaction is dependent on concentration binding protein (DBP). However, molecular basis synergistic action DBP present unknown. We report here increases affinity its site origin. did not, however, increase another sequence-specific protein, EBP1, recognition site. Other single-stranded proteins could not substitute increasing In addition, was found alter kinetics NFI, both by rate association decreasing dissociation with template. co-operativity between demonstrated template, human (FIB2), suggesting this interaction general occurrence restricted