Evolutionary trace for prediction and redesign of protein functional sites.

摘要: The evolutionary trace (ET) is the single most validated approach to identify protein functional determinants and target mutational analysis, engineering drug design relevant sites of a protein. It applies entire proteome; its predictions come with reliability score; results typically reach significance in families 20 or more sequence homologs. In order hot spots, ET scans multiple alignment for residue variations that correlate major divergences. case studies this enables selective separation, recoding, mimicry and, on large scale, specific function based motifs built from select ET-identified residues. therefore an accurate, scalable efficient method molecular direct their rational perturbation therapeutic purposes. Public servers are located at: http://mammoth.bcm.tmc.edu/.