Purification and characterization of Tet(M), a protein that renders ribosomes resistant to tetracycline.
作者： V Burdett
DOI: 10.1016/S0021-9258(18)49928-0
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摘要: The tet(M) tetracycline resistance gene has been found in a wide variety of clinically important bacteria. It shown previously (Burdett, V. (1986) J. Bacteriol. 165, 564-569) that the product mediates at level protein synthesis as judged by vitro assay. Using this assay, large amounts were purified from an Escherichia coli overproducer expressing under control T7 promoter. activity consists single polypeptide molecular weight 68,000 sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and was confirmed to be amino-terminal sequence analysis. Purified Tet(M) associated ribosome-dependent GTPase with specific being similar corresponding elongation factor G. Since also displays substantial homology G throughout its length, may function analog factor.
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