Unified description of electrophoresis and diffusion for DNA and other polyions.

摘要: The electrophoretic mobilities and diffusion coefficients of single- double-stranded DNA molecules up to 50 000 bases or base pairs in size have been analyzed, using either measured by capillary electrophoresis taken from the literature. Einstein equation suggests that (μ) (D) should be related expression μ/D = Q/kBT, where Q is charge polyion (Q zeo, z number charged residues eo fundamental electronic charge), kB Boltzmann's constant, T absolute temperature. If this were true, ratio μ/zD a constant equal eo/kBT (39.6 V-1) at 20 °C. However, decreases with an increase molecular weight for both DNAs. are better described modified μ/NmD eo/kBT, N repeat units (bases pairs) ...