A Burkholderia sacchari cell factory: production of poly-3-hydroxybutyrate, xylitol and xylonic acid from xylose-rich sugar mixtures.

摘要: Abstract Efficient production of poly-3-hydroxybutyrate (P(3HB)) based on glucose-xylose mixtures simulating different types lignocellulosic hydrolysate (LCH) was addressed using Burkholderia sacchari, a wild strain capable metabolizing both sugars and producing P(3HB). Carbon catabolite repression avoided by maintaining glucose concentration below 10 g/L. Xylose concentrations above 30 g/L were inhibitory for growth production. In fed-batch cultivations, pulse size feed addition rate controlled in order to reach high productivities efficient sugar consumptions. High xylose uptake P(3HB) productivity attained with glucose-rich (glucose/xylose ratio the feed = 1.5 w/w) feeding rates, while xylose-richer feeds (glucose/xylose = 0.8 w/w), lower is robust strategy avoid build-up medium. Xylitol observed medium 30–40 g/L. With featuring even glucose/xylose ratios, i.e. (glucose/xylose = 0.5), xylonic acid (a second byproduct) produced. This first report ability sacchari produce xylitol acid.