Antitumor Immune Response of Human Peripheral Blood Lymphocytes Coengrafted with Tumor into Severe Combined Immunodeficient Mice

摘要: Here, it is established that human peripheral blood lymphocytes (HuPBLs), injected s.c. with a lung tumor into severe combined immunodeficient (SCID) mice, engraft and display antitumor cytotoxic activity. Initial studies used HuPBLs from normal donors an allogeneic cell line derived biopsy tissue of patient squamous carcinoma the lung. Evidence HuPBL activity was revealed by dose-dependent suppression xenograft. Tumor shown to be dependent upon both CD8+ T cells CD56+ natural killer in donor HuPBLs. By titrating SCID mice without cytokines, interleukin (IL)-12 enhanced HuPBL-mediated IL-2 had synergistic effect IL-12 enhancement cytotoxicity. Subsequent cancer patient's PBLs also suppress growth (autologous) when coinjected mice. The immunity mediated cells. data presented here indicate coengraftment represents viable model which study (and periodically monitor) patients' immune responses their tumors for extended periods time suggest this SCID/Winn assay could evaluate novel immunotherapeutic approaches, such as bolus injections cytokine gene therapy, or vaccination strategies treatment cancer.