Affinity Chromatography and Importance in Drug Discovery

摘要: Affinity chromatography which is known as a liquid chromatographic technique for separation and analysis of biomolecules based on their biological functions or individual structures has become increasingly important useful method in pharmaceut‐ ical science, biochemistry, biotechnology environmental science recent years [1]. This especially the most specific effective protein purification [2]. Separation highly interactions between two molecules, such enzyme substrate. These interactions, are typically reversible, used by placing one interacting referred to affinity ligand, onto solid matrix create stationary phase while target molecule mobile [3]. Any component can be ligand purify its respective binding partner. Some typical frequently chromatography, given as;