Deregulation of Dorsoventral Patterning by FGF Confers Trilineage Differentiation Capacity on CNS Stem Cells In Vitro

摘要: The CNS is thought to develop from self-renewing stem cells that generate neurons, astrocytes, and oligodendrocytes. Other data, however, have suggested astrocytes oligodendrocytes are generated separate progenitor populations. To reconcile these observations, we prospectively isolated progenitors do or not express Olig2, an oligodendrocyte bHLH determination factor. Both Olig2− Olig2+ can behave as tripotential (CNS-SCs) in vitro. Growth FGF-2 causes induction of Olig2 the former population, permitting differentiation; extinction latter permits astrocyte differentiation. by mediated, part, via endogenous Sonic Hedgehog. These data indicate clonogenic competence reflects a deregulation dorsoventral patterning during expansion vitro, raising question whether such trifatent actually exist vivo.